Studies on the Freezability of Kundhi Buffalo Bull Semen

نویسندگان

  • Z. U. Rehman
  • M. U. Samo
  • T. A. Qureshi
  • S. Khan
  • M. S. Qureshi
  • F. A. Khan
چکیده

This study was conducted to assess the post thaw quality of Kundhi buffalo semen diluted in tris based diluents. After collection the ejaculates were pooled and checked for color, volume, pH, mass activity, progressive linear motility% (PLM), and intactness of cell membrane. The semen qualifying these tests was divided into four aliquots A, B, C and D, diluted with tris based diluents; the last three diluents containing sugar types glucose, fructose and lactose respectively. Aliquot A was without sugar and severed as control. These were frozen, stored for 24 hrs, thawed and incubated at 35C for 5 hrs. The incubated semen aliquots were examined for PLM and membrane integrity of sperm cells with phasecontrast microscopy and HOST procedures. It was observed that all the ejaculates were creamy white in color. The mean (±SEM) volume, mass activity, sperm concentration, PLM, and intactness of cell membrane were 1.70 (±0.09) ml, 3.22 (±0.074), 1.58 (±0.136) x 10/ml, 64.53 (±0.757) % and 55.63 (±0.945)% respectively for fresh semen. A significant difference was observed in pH values of the fresh semen samples among the bulls. There was a significant decrease in the number of motile cells and the cells with intact membrane after thawing and incubation of the semen at 35C. Addition of fructose in the diluents was found to be superior in maintaining higher PLM and intactness of membrane after thawing and incubation. The objective method, Osmotic resistance test (ORT) was found to be the useful parameter for assessment of in-vitro fertility of Kundhi buffalo bull semen.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Factors affecting the quality of cryopreserved buffalo (Bubalus bubalis) bull spermatozoa.

Storage of buffalo (Bubalus bubalis) bull semen in the cryopreserved state is discussed in this article. Fertility rate in buffalo following artificial insemination with frozen-thawed semen is reviewed. To better understand the freezability of bubaline spermatozoa, the available data on biochemical components and the activity of specific enzymes of semen/spermatozoa are given. Moreover, the maj...

متن کامل

O-12: Studies on Sequestration of PDC-109 Protein on Cryodamage and In Vitro Fertility of Crossbred Bull Spermatozoa

Background: Plasma membrane of the spermatozoa interacts with and is altered by abundant seminal PDC-109 present in their immediate milieu in vivo with damaging effect in a time- and concentration- dependent manner. Therefore we hypothesized that sequestration of detrimental protein from ejaculates would be beneficial for cryopreservation of sperm cells. To this aim we evaluated the effect of s...

متن کامل

Sperm Characteristics in Cryopreserved Buffalo Bull Semen and Field Fertility

Semen quality and its relationship to fertility are major concern in animal production. The aim of this study was to assess the relationship between frozen thawed sperm characteristics and fertility following in vivo fertility through Artificial Insemination(AI). Semen samples were collected from four buffalo bulls. Semen volume and sperm concentration appeared to be significantly different (P0...

متن کامل

Glutathione-supplemented tris-citric acid extender improves the post-thaw quality and in vivo fertility of buffalo (Bubalus bubalis) bull spermatozoa.

In this study we evaluated the effects of semen extender supplementation with different concentrations of glutathione (GSH) on buffalo (Bubalus bubalis) bull sperm motility, plasma membrane integrity, viability and DNA integrity as well as in vivo fertility. Semen from three Nili-Ravi buffalo bulls was collected, and qualified semen ejaculates (n=18) were split into five aliquots for dilution (...

متن کامل

P-117: The In Vitro Effect of Leptin on Semen Quality of Water Buffalo (Bubal Us Bubalis) Bulls

Background: Several studies have recently investigated the role of leptin, the polypeptide hormone secreted primarily by white adipose tissue, in the reproduction of rodents, humans, and domestic animals. Materials and Methods: To investigate the in vitro effect of leptin on buffalo semen quality in equilibrated and frozen thawed semen, five healthy buffalo bulls (5 ejaculates from each bull) w...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2012